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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
05/01/1994 |
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Data da última atualização: |
05/01/1994 |
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Autoria: |
SYLVIA, D. M.; JARSTFER, A. G. |
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Afiliação: |
Soil Science Department, 2171 McCarty Hall, University of Florida, Gainesville, Florida 326211-0151. |
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Título: |
Sheared-Root inocula of vesicular-Arbuscular mycorrhizal fungi |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Applied environmental microbiology, jan 1992, p. 229-232. |
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Idioma: |
Inglês |
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Conteúdo: |
For efficient handling, vesicular-arbuscular mycorrhizal fungi should by processed into small nad uniform inocula; however, processing can reduce the inoculum density. In this article we describe the preparation and use of sheared-root inocula of Glomus spp. in which inoculum densities were increased during processing. Our objectives were to determine inoculum viability and density after shearing and to ascertain if the sheared inocula could be pelletized or used with a gel carrier. Root samples were harvested from aeroponic cultures, blotted dry, cut into 1-cm lengths, and sheared in a food processor for up to 80 s. After shearing, the inoculum was washed over sieves, and the propagule density in each fraction was determined. Sheared inocula were also encapsulqted in carrageenan or used in a gel carrier. Shearing aeroponically produced root inocula reduced particle size. Propagule density increased with decreasing size fraction down to a size of 63 um, after which propagule density decreased. The weighted-average propagule density the inoculum was 135,380 propagules g (dry weght) of sheared root material-1. Sheared roots were encapsulated successfully in carrageenan, andthe gel served as an effective carrier. Aeroponic root inoculum was stored dry at 4graus C for 23 months without significant reduction in propagule density; however, this material was not appropriate for shearing. Moist roots, useful for shearing, began to lose propagule density after 1 month os storage. Shearing proved to be an excellent method MenosFor efficient handling, vesicular-arbuscular mycorrhizal fungi should by processed into small nad uniform inocula; however, processing can reduce the inoculum density. In this article we describe the preparation and use of sheared-root inocula of Glomus spp. in which inoculum densities were increased during processing. Our objectives were to determine inoculum viability and density after shearing and to ascertain if the sheared inocula could be pelletized or used with a gel carrier. Root samples were harvested from aeroponic cultures, blotted dry, cut into 1-cm lengths, and sheared in a food processor for up to 80 s. After shearing, the inoculum was washed over sieves, and the propagule density in each fraction was determined. Sheared inocula were also encapsulqted in carrageenan or used in a gel carrier. Shearing aeroponically produced root inocula reduced particle size. Propagule density increased with decreasing size fraction down to a size of 63 um, after which propagule density decreased. The weighted-average propagule density the inoculum was 135,380 propagules g (dry weght) of sheared root material-1. Sheared roots were encapsulated successfully in carrageenan, andthe gel served as an effective carrier. Aeroponic root inoculum was stored dry at 4graus C for 23 months without significant reduction in propagule density; however, this material was not appropriate for shearing. Moist roots, useful for shearing, began to lose propagule density after 1 month os storage. She... Mostrar Tudo |
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Palavras-Chave: |
Glomus intraradix; Glomus spp; Raizes; Solos Doenca. |
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Categoria do assunto: |
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Marc: |
LEADER 02025naa a2200181 a 4500
001 1646406
005 1994-01-05
008 1992 bl --- 0-- u #d
100 1 $aSYLVIA, D. M.
245 $aSheared-Root inocula of vesicular-Arbuscular mycorrhizal fungi
260 $c1992
520 $aFor efficient handling, vesicular-arbuscular mycorrhizal fungi should by processed into small nad uniform inocula; however, processing can reduce the inoculum density. In this article we describe the preparation and use of sheared-root inocula of Glomus spp. in which inoculum densities were increased during processing. Our objectives were to determine inoculum viability and density after shearing and to ascertain if the sheared inocula could be pelletized or used with a gel carrier. Root samples were harvested from aeroponic cultures, blotted dry, cut into 1-cm lengths, and sheared in a food processor for up to 80 s. After shearing, the inoculum was washed over sieves, and the propagule density in each fraction was determined. Sheared inocula were also encapsulqted in carrageenan or used in a gel carrier. Shearing aeroponically produced root inocula reduced particle size. Propagule density increased with decreasing size fraction down to a size of 63 um, after which propagule density decreased. The weighted-average propagule density the inoculum was 135,380 propagules g (dry weght) of sheared root material-1. Sheared roots were encapsulated successfully in carrageenan, andthe gel served as an effective carrier. Aeroponic root inoculum was stored dry at 4graus C for 23 months without significant reduction in propagule density; however, this material was not appropriate for shearing. Moist roots, useful for shearing, began to lose propagule density after 1 month os storage. Shearing proved to be an excellent method
653 $aGlomus intraradix
653 $aGlomus spp
653 $aRaizes
653 $aSolos Doenca
700 1 $aJARSTFER, A. G.
773 $tApplied environmental microbiology, jan 1992, p. 229-232.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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